Itu perbedaan antara fluoresensi mikroskop dan laser confocal mikroskop
Berbeda prinsip
1. Fluorescent microscope: It uses ultraviolet light as the light source to illuminate the object being tested, causing it to emit fluorescence, and then observe the shape and location of the object under the microscope.
2. Laser confocal microscope: On the basis of fluorescence microscope imaging, a laser scanning device is installed to excite the fluorescence probe using ultraviolet or visible light.
Berbeda karakteristik
1. Fluorescence microscope: used to study the absorption, transportation, distribution, and localization of intracellular substances. Some substances in cells, such as chlorophyll, can emit fluorescence after being exposed to ultraviolet radiation; Some substances themselves may not emit fluorescence, but if stained with fluorescent dyes or fluorescent antibodies, they can also emit fluorescence under ultraviolet radiation.
2. Laser confocal microscope: Using a computer for image processing to obtain fluorescence images of the internal microstructure of cells or tissue, and to observe physiological signals such as Ca2+, pH value, membrane potential, and changes in cell morphology at the subcellular level.
1. Fluoresensi mikroskop% 3a Fluoresensi mikroskop adalah a fundamental alat in imunofluoresensi sitokimia. It is tersusun dari utama komponen seperti sebagai cahaya sumber, filter pelat sistem, dan optik sistem. It is penggunaan dari a tertentu panjang gelombang dari cahaya untuk menggairahkan the spesimen dan memancarkan fluoresensi, yang adalah diperbesar melalui an objektif dan lensa mata sistem untuk mengamati the fluoresensi gambar dari spesimen.
2. Laser confocal microscopy: Laser scanning confocal microscopy technology has been used for research on cell morphology localization, three-dimensional structural recombination, dynamic change processes, and provides practical research methods such as quantitative fluorescence measurement and quantitative image analysis. Combined with other related biotechnology, it has been widely applied in molecular cell biology fields such as morphology, physiology, immunology, genetics, etc.
